GABA A receptor alpha 1, Synaptic Systems, 224 203
What do reviewers generally think about 224 203 from Synaptic Systems?
Researchers report the antibody performs well for detecting GABA A receptor alpha 1 in murine primary cortical neuron lysates and mouse hippocampal cryo sections, with clear signal and low background at 1:1000. One reviewer points out that a very short exposure was needed for lysate bands, while another notes that 1:5000 may give an even better result within the manufacturer’s suggested range. No complaints are mentioned.
Information about this antibody
Vendor notes
User Reviews (2)
Review of: GABA A receptor alpha 1, Synaptic Systems, 224 203 by Anders Dalby
This antibody works great to detect GABA-A receptor subunit alpha 1 in lysates from murine primary neurons (cortical). A very short exposure time were needed to develop bands using this antibody.
Dilution 1:1000
0 useful
0 not usefulReview of: GABA A receptor alpha 1, Synaptic Systems, 224 203 by Simon Mølgaard Jensen, Aarhus University
This antibody was tested on 15 um thick hippocampal cryo sections from a perfusion fixed mouse. Prior to immunohistochemistry, the tissue was target retrieved using DAKO antigen retrieval s1699 according to protocol from the manufacturer.
This antibody works excellent for detecting expression of GABA(A) receptor subtype alpha 1 in the hippocampus.
This was tested at 1:500 and1:1000. At 1:1000 a clear signal was seen with low background. The manufacturer suggest using a dilution between 1:1000 to 1:5000 so an even better result may be obtained using 1:5000.
Comments
0 useful 0 not useful
Comments