NMDA receptor 2A, Alomone Labs, AGC-002

Our team has carried out deep research on the published scientific literature to identify papers using AGC-002 followed by extraction and validation of the available data to assess its performance according to pAbmAbs rating criteria.
AGC-002 has been applied across multiple experimental systems, including WB, IP, IHC, and live-cell surface labeling. The antibody consistently detects GluN2A at the expected molecular weight, yields clear tissue staining, and supports advanced applications such as quantum-dot tracking and live surface staining. Published studies report good signal-to-noise and appropriate technical controls (IgG immunoprecipitation controls, secondary-only staining, clear bands at expected MW). However, no publications to date show direct GluN2A knockout tissue validation or antigen pre-adsorption experiments with AGC-002. Specificity is therefore supported indirectly by orthogonal functional and genetic perturbations, rather than by gold-standard KO or peptide-blocking tests.
WB: AGC-002 detects a clear GluN2A band at ~180 kDa. IgG controls were clean in IP experiments. Reproducible across different cell and tissue types. Lacks KO or pre-adsorption confirmation.
IHC: AGC-002 provides robust staining with low background, even at high dilutions. Secondary-only controls show specificity. Works in human and rodent tissue, but no KO or peptide-blocked IHC data have been published.
Live imaging: effective for labeling extracellular GluN2A in live neurons, enabling cluster quantification and single-molecule tracking. Orthogonal functional assays (electrophysiology, genetic perturbations) support biological relevance of the labeling. No GluN2A KO or peptide-competition data shown in these studies.
We highly encourage other scientists to share their observations.

Selected references:

GluN3A subunit tunes NMDA receptor synaptic trafficking and content during postnatal brain development

Rabphilin-3A undergoes phase separation to regulate GluN2A mobility and surface clustering | Nature Communications

Functional NMDA receptors are expressed by human pulmonary artery smooth muscle cells | Scientific Reports

I used this antibody on 12 div cultured primary hippocampam neurons. The neurons were immunstained according to the protocol in Molgaard et al

https://f1000research.com/articles/3-242/v3

The antibody was used at a dilution of 1:100. Excellent for detecting NR2A by ICC.

Alomone Labs Anti-NMDA Receptor 2A (GluN2A) (extracellular)
Excellent staining, used pre-adsorbed secondary Antibodies form abcam.
Testes serveal GluN2A, this is the first one working 🙂

Works very reliable