trimethyl-Histone H3 (Lys27)/H3K27me3, Millipore, 07-449
What do reviewers generally think about 07-449 from Sigma-Aldrich?
Researchers report using this antibody for western blotting, immunostaining of Drosophila imaginal discs, and ChIP. Reported conditions include 1:500 for western blotting and immunostaining, 1:2000 for western blotting, and 2 µg per IP for ChIP. One reviewer points out that the antibody can be used for western blotting at both 1:500 and 1:2000, suggesting some flexibility in dilution. No performance complaints are mentioned.
Information about this antibody
Alt. Targets
Vendor
Catalog No
07-449
Application
Clonality
pAb
Origin
Rabbit
Reactivity
Human, Mouse
Conjugation
N/A
Product Link
N/A
Vendor notes
No vendor notes available for this antibody.
User Reviews (4)
Review of: trimethyl-Histone H3 (Lys27)/H3K27me3, Millipore, 07-449 by Bing Zhang initiative
Used for western blotting in the dilution of 1:500.
Bing Zhang initiative103 reviews
0 useful
0 not usefulReview of: Trimethyl-histone, merckmillipore, 07-449 by Philip Yung, Cavalli lab, CNRS
This antibody was used for immunostaining of Drosophila Imaginal Discs.
Dilution 1:500
Ref: Yung et al., 2015, Cell Reports 11, 1437–1445, June 9, 2015
Comments
0 useful 0 not usefulReview of: Trimethyl-histone, merckmillipore, 07-449 by Britt R. Ardal (THJ lab, AU)
This antibody is used for ChIP at 2µg/IP
Comments
0 useful 0 not usefulReview of: Trimethyl-histone, merckmillipore, 07-449 by Britt R. Ardal (THJ lab, AU)
This antibody is used for WB diluted 1:2000
Comments
0 useful 0 not useful
Comments