IgG (Fc region), Sigma-Aldrich, A2554
What do reviewers generally think about A2554 from Sigma-Aldrich?
Researchers report the goat anti-mouse IgG-HRP antibody performs well in ELISA and western blotting for detecting diabodies, giving specific, high signal with very low background at 1:1000, 1 ug/mL, or 0.20 ug/mL. One reviewer points out that it can be used together with anti-FLAG and anti-myc antibodies for bispecific diabody detection. No complaints are mentioned.
Information about this antibody
Vendor notes
User Reviews (2)
Review of: IgG (Fc region), Sigma-Aldrich, a2554 by Kasper Mølgaard Jensen, Aarhus University
Anti-Mouse IgG–Peroxidase antibody produced in goat, Sigma Aldrich
I have used GAM-HRP for detection in ELISA of bound diabodies expressed from HEK 293T cells. It works very good in ELISA, giving specific and high signals.
It was used diluted 1:1000
0 useful
0 not usefulReview of: IgG (Fc region), Sigma-Aldrich, a2554 by Kasper Mølgaard Jensen, Aarhus University
Used in western blotting at a concentration of 0.20 ug/mL with good results. Very low background observed and specific detection.
Used in ELISA to detect bispecific diabodies together with anti-FLAG (Sigma-Aldrich) and anti-myc (Sigma-Aldrich). Very good and specific results were obtained with very low background. Used at a concentration of 1 ug/mL.
Comments
0 useful 0 not useful
Comments