Beta-actin, Cell Signaling Technology, 3700
What do reviewers generally think about 3700 from Cell Signaling Technology?
Researchers consistently report that Beta-actin CST 3700 performs well for western blotting, with successful use in hESCs, Huh-7, and BHK-21 cells at dilutions from 1:1000 to 1:5000. One reviewer points out that at 1:20,000 it is very sensitive and works superbly, including for double immunoblotting with a rabbit antibody as a loading control. No negative performance issues are mentioned.
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User Reviews (3)
Review of: Beta-actin, Cell Signaling Technology, 3700 by Patricia Shigunov, Fiocruz Paraná
Great for western blotting (1:1000) of hESCs.
For more information see Silva et al. PLOS One 2020, “Effects of PUMILIO1 and PUMILIO2 knockdown on cardiomyogenic differentiation of human embryonic stem cells culture”.
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0222373#sec002
0 useful
0 not usefulReview of: Beta-actin, Cell Signaling Technology, 3700 by Milly Ming Ju Choy, professor Ooi Eng Eong lab, National University of Singapore
Great antibody for western blotting of Huh-7 and BHK-21 cells. Dilution: 1:5000
For more information see Choy et al. Cell Reports 2020, “A Non-structural 1 Protein G53D Substitution Attenuates a Clinically Tested Live Dengue Vaccine”.
https://www.sciencedirect.com/science/article/pii/S2211124720305660#!
Comments
0 useful 0 not usefulReview of: Beta-actin, Cell Signaling Technology, 3700 by Frédéric Couture, Université de Sherbrooke, Canada
Dilution 1/20,000
Works superbly in WB, very sensitive. Perfect for double immunoblotting (combined with a rabbit to serve as a loading control)
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