Poly-Histidine, Sigma-Aldrich, A7058
What do reviewers generally think about A7058 from Sigma-Aldrich?
Researchers report that Poly-Histidine, Sigma-Aldrich, A7058 performs well in ELISA and Western blotting for detecting trimerbodies, bispecific diabodies from HEK 293T cells, and antibodies from E. coli. The consensus is that it provides specific detection with low or slight background at a 1:4000 dilution. One reviewer points out that it gave OK results in Western blotting with a little background at 1:4000.
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User Reviews (3)
Review of: Poly-Histidine, Sigma-Aldrich, A7058 by Kasper Mølgaard Jensen
PEROXIDASE ANTI-POLYHISTIDINE, MOUSE MAB, SIGMA-ALDRICH, A7058
Used in ELISA to detect trimerbodies.
Used at 1:4000 dilution (around [2ug/mL]).
0 useful
0 not usefulReview of: Poly-Histidine, Sigma-Aldrich, A7058 by Kasper Mølgaard Jensen, Aarhus University
Used in Western Blotting to detect bispecific diabody expressed in HEK 293T cells with OK results. Specific detection with a little background.
Used diluted 1:4000
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0 useful 0 not usefulReview of: Poly-Histidine, Sigma-Aldrich, A7058 by Laura Falkensteen Overgaard
Used in western blot against antibodies expressed in E.Coli with good results. Low background was observed.
Used with a dilution of 1:4000.
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0 useful 0 not useful
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