GFP, Abcam, ab290
What do reviewers generally think about ab290 from Abcam?
Researchers consistently report excellent performance of GFP, Abcam, ab290 for immunohistochemistry and CHIP, including Drosophila, rat brain, and mouse cryosections, with good visualization of GFP overexpression. One reviewer points out that a 1:30,000 dilution in 2.5% Triton/KPBS with overnight incubation at room temperature works well for free-floating PFA-fixed rat brain sections. Other successful protocols use 1:500 dilution in PBS with 0.1% Tween or 1:500 for thin mouse cryosections. No complaints are mentioned.
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User Reviews (3)
Review of: GFP, Abcam, ab290 by Uwe Töpfer
Used for immunohistochemistry and CHIP with excellent results. Dilution: 1:500 in PBS with 0.1% Tween ON at RT.
Töpfer, U., Bischoff, M.C., Bartkuhn, M. et al. Serpent/dGATAb regulates Laminin B1 and Laminin B2 expression during Drosophila embryogenesis. Sci Rep 9, 15910 (2019).
https://doi.org/10.1038/s41598-019-52210-9
0 useful
0 not usefulReview of: GFP, Abcam, ab290 by Gitte Ulbjerg Toft, Aarhus University
Used for immunohistochemistry on free floating PFA fixed rat brain sections.
Very good antibody to visualize GFP overexpression in rat brain.
Working dilution: 1:30.000 in 2,5% Triton/KPBS, overnight incubation at RT.
Comments
0 useful 0 not usefulReview of: GFP, Abcam, ab290 by Marie-Louise Holm, AU
This antibody is excellent for immunohistochemistry on thin mouse cryo sections (10μm).
Dilution: 1:500
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