NOP56, Thermo Fischer Scientific, A302-720
What do reviewers generally think about A302-720 from Thermo Fisher Scientific?
Researchers report that NOP56, Thermo Fischer Scientific, A302-720 performs well for Western blotting and immunofluorescence. For Western blotting at 1:5000 in 5% milk overnight at 4°C, it gives a strong, clear ~70 kDa band with no unspecific binding. One reviewer points out that it can be reused. For immunofluorescence on Hek293 cells at 1:500, it produced clear signal with an Alexa 594 secondary at 1:1000. The antibody targets the c-terminus of NOP56, and no major complaints are mentioned.
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Vendor notes
User Reviews (3)
Review of: NOP56, Thermo Fischer Scientific, A302-720 by Britt R. Ardal (THJ lab, Aarhus University)
Used for immune fluorescence on Hek293 cells 1:500.
signal was clear and easy to do microscopy on when using Alexa 594 (texas red) secondary antibody in 1:1000.
0 useful
0 not usefulReview of: NOP56, Thermo Fischer Scientific, A302-720 by Britt R. Ardal (THJ lab, Aarhus University)
Used for Western blotting in 1:5000 in 5% milk o/n on rotation at 4 degrees.
Signal was very good and clear with no unspecific binding.
Antibody targets the c-terminus of NOP56.
Comments
0 useful 0 not usefulReview of: NOP56, Thermo Fischer Scientific, A302-720 by Ewa Molska (THJ lab, Aarhus University)
The antibody detects a ~70 kDa WB band. Gives strong clean signal (diluted 1:5000) and can be reused
Comments
0 useful 0 not useful
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