GABA A receptor alpha 2, Abcam, ab72445
What do reviewers generally think about ab72445 from Abcam?
Researchers generally report ab72445 performs well in western blot at 1:1000 on mouse frozen tissue and primary hippocampal neurons, giving a specific ~51 kDa band with little or no background. One reviewer points out that IHC at 1:250 gave a clear signal with very low background, while western blot also showed an unspecific band alongside the expected clear band. Overall consensus is positive for specificity and low background in western blot and IHC, with a minor non-specific western blot band noted.
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User Reviews (4)
Review of: GABA A receptor alpha 2, Abcam, ab72445 by Abdel-Rahman Al-Absi
Anti-GABA A Receptor alpha 2 antibody (ab72445)
I have used this antibody to do western blot on mouse frozen tissue. I have incubated with this antibody (1:1000) at 4 °C over night, then with secondary antibody at room temperature for 1 hour. The antibody gives nice band and almost no background.
0 useful
0 not usefulReview of: GABA A receptor alpha 2, Abcam, ab72445 by Signe Petersen, Aarhus University
Anti-GABA A Receptor alpha 2 antibody (ab72445)
I used this antibody in a dilution of 1:1000 for a western blot of BDNF-stimulated primary hippocampal neurons with great results. No unspecific bands were detected.
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0 useful 0 not usefulReview of: GABA A receptor alpha 2, Abcam, ab72445 by Lars Meinertz Byg
I used this antibody for westernblotting in a 1:1000 dilution in lanes with 25 ug protein and got a very specific signal around 51 kDA.
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0 useful 0 not usefulReview of: GABA A receptor alpha 2, Abcam, ab72445 by Simon Mølgaard Jensen
This was used for IHC and Western blot with great results. Gave a clear signal with very low background when doing IHC. When used for Western blot, an unspecific band was seen but also a clear band at the expected size.
IHC 1:250
WB 1:1000
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