LDL receptor, Novus Biologicals, NBP1-06709
What do reviewers generally think about NBP1-06709 from Novus Biologicals?
Researchers report that NBP1-06709 works for Western blotting and immunofluorescence. In mouse liver membrane WB at 1:1000, it detected a band just above 98 kDa, expected for LDLR, which was down-regulated after PCSK9 injection, but numerous bands appeared and the LDLR band was not the most prominent. One reviewer points out that re-incubation of the upper part of the blot increased the LDLR band intensity. For IF at 1:100, it gave intense staining in HepG2 cells, with a more vesicular pattern in PCSK9-transfected cells.
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User Reviews (2)
Review of: LDL receptor, Novus Biologicals, NBP1-06709 by Camilla Gustafsen, Aarhus University
I have used this antibody diluted 1:1000 for WB of mouse liver membrane preparations (40 µg total protein loaded on gel). Numerous bands were seen (see full Western blot above).
The highest band is of expected size just above marker band size 98 kDa. The band is down-regulated in animals injected with PCSK9, however, it is not the most prominent of the detected bands.
Re-incubation of the upper part of the WB increased the LDLR band intensity (shown above).
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0 not usefulReview of: LDL receptor, Novus Biologicals, NBP1-06709 by Laura Tvilling Poulsen (Aarhus University)
Anti-LDLR, NBP1-06709, rabbit.
This antibody gave an intense IF staining in both HepG2 cells transfected with PCSK9 and untransfected HepG2 cells. The staining was more vesicular in transfected cells.
Dilution 1:100
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