FLAG, Sigma-Aldrich, F1804
What do reviewers generally think about F1804 from Sigma-Aldrich?
Researchers generally report that FLAG, Sigma-Aldrich, F1804 performs well for detecting FLAG-tagged proteins in Western blot, immunofluorescence, ELISA, ChIP, and IP, often with low background and good specificity. Reported useful conditions include 1:500 for immunostaining, 1:5000 or 1:4000 for Western blot, 1:1000 for WB/IF, 0.2 µg/mL for ELISA, and 2 µg for ChIP. One reviewer points out that, although it detects the FLAG epitope, high background made it unusable in their hands.
Information about this antibody
Vendor notes
User Reviews (9)
Review of: FLAG, Sigma-Aldrich, F1804 by Wang, Chinese Academy of Sciences
Dilution 1:500 for immunostaining
0 useful
0 not usefulReview of: FLAG, Sigma-Aldrich, F1804 by Bing Zhang initiative
Does detect the flag epitope, but such high background as to be unusable.
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0 useful 0 not usefulReview of: FLAG, Sigma-Aldrich, F1804 by Margie Sutton, University of Texas MD Anderson Cancer Center
WB dilution 1:5000
For more information see Sutton et al. Cell Reports 2019, “DIRAS3 (ARHI) Blocks RAS/MAPK Signaling by Binding Directly to RAS and Disrupting RAS Clusters”
https://www.sciencedirect.com/science/article/pii/S221112471931527X#sec5
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0 useful 0 not usefulReview of: FLAG, Sigma-Aldrich, F1804 by Rachel L. Flynn, Boston University School of Medicine, US
This antibody was used for ChIP (2µg).
Reference K. E. Cox et al., Cell Reports, 9 February 2016
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0 useful 0 not usefulReview of: FLAG, Sigma-Aldrich, F1804 by Cinzia Tesauro, Aarhus University
I have used this antibody for many years to verify the expression and purity of flag-tagged proteins. Never had problems of background.
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0 useful 0 not usefulReview of: FLAG, Sigma-Aldrich, F1804 by Jakob Vejby Larsen
I have used this antibody in 293 cells transfected with a protein containing a FLAG-tag in the N-terminus. It works great in both WB and IF (dilution 1:1000)
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0 useful 0 not usefulReview of: FLAG, Sigma-Aldrich, F1804 by Kasper Mølgaard Jensen
anti-FLAG® M2 mouse mAb Sigma-Aldrich F1804
I have used anti-FLAG in western blotting to detect bispecific diabodies (along with anti-myc), and it works perfectly. Low background and very specific detection of FLAG taged antibodies was observed. Furthermore it was possible to detect a difference in expression levels of the two chains of the diabody.
Used as 1:4000 [0.25ug/mL].
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0 useful 0 not usefulReview of: FLAG, Sigma-Aldrich, F1804 by Kasper Mølgaard Jensen, Aarhus University
I have used this in ELISA and have had great results, very low background and high specificity. Used in concentration of 0.2ug/mL.
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0 useful 0 not usefulReview of: FLAG, Sigma-Aldrich, F1804 by Bjarke Primdal-Bengtson, Aarhus University
I have used this with great succes for quantifying expression of FLAG-tagged Rab27B using Western blot. Also, I have looked upon binding partners to FLAG-tagged Rab27B through IP.
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0 useful 0 not useful
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