SorCS1, Novus Biologicals, nbp1-86096
What do reviewers generally think about nbp1-86096 from Novus Biologicals?
Researchers report that SorCS1, Novus Biologicals, nbp1-86096 performs well in immunostaining of SorCS1-expressing 293 cells with high signal-to-noise at 1:200. It detects mouse and human SorCS1 in Western blots of CHO cells stably expressing variants at 1:1000, but fails to detect endogenous murine SorCS1 in p0 and adult mouse brain. It supports human SorCS1 immunoprecipitation at 1 µg/IP and Western blotting. One reviewer points out that it is marginally less efficient than HPA011948 or AF3457 and recognizes only the C-terminal region, not the N-terminal part.
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User Reviews (3)
Review of: SorCS1, Novus Biologicals, nbp1-86096 by Peter Lund Ovesen
Works excellent in immunostainings of sorcs1-expressing 293 cells. High signal to noise. (1:200)
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0 not usefulReview of: SorCS1, Novus Biologicals, nbp1-86096 by Peter Lund Ovesen
Detects both mouse and human SorCS1 in Wetsern blots for samples of CHO cells stable expressing each variant of SorCS1.
On the other hand, it does not work for detecting endogenous murine SorCS1 from brains of p0 and Adult mice in Western blotting. A SorCS1 knockout brain were used as negative reference. Cells expressing SorCS1 as positive ref.
Concentration 1:1000
Comments
0 useful 0 not usefulReview of: SorCS1, Novus Biologicals, nbp1-86096 by Mads Kjolby, Aarhus University
Anti-SorCS1 NBP1-86096 works for IP of human SorCS1, 1ug/IP (50ul Gammabind Sepharose-G = 1 IP). The antibody is almost as good as HPA011948 or AF3457, but may by marginally less efficient.
It also works for Western blotting for detection of human sorCS1.
The antibody is raised against aa996-1095. I can confirm it does not detect the N-terminal part, but only the c-terminal part.
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