LPL, ImmuQuest, IQ449
What do reviewers generally think about IQ449 from ImmuQuest?
Researchers report LPL, ImmuQuest, IQ449 works for detecting LPL in mouse astrocyte and cortical glia preparations. One reviewer points out that it was used for IP of LpL from primary mouse astrocytes at 1 µg/IP. Another reviewer finds IF staining specific and sensitive, marking vesicular structures in mouse cortical glia, with permeabilization in PBS+0.1% Triton X-100, blocking in PBS+10% FBS, and a 2 µg/ml dilution. No complaints are mentioned.
Information about this antibody
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User Reviews (2)
Review of: LPL, ImmuQuest, IQ449 by Camilla Gustafsen, Aarhus University
anti-LpL, mouse mAb clone 4G33. I have used this antibody for IP of LpL from primary mouse astrocytes (1µg/IP)
0 useful
0 not usefulReview of: LPL, ImmuQuest, IQ449 by Simon Glerup, Mayo Clinic
This antibody is raised against lipoprotein lipase (LPL), which is known to be expressed by cortical astrocytes.
The staining seems specific, sensitive, and marks vesicular structures in cultures of mouse cortical glia.
Cells were permeabilized in PBS+0.1% TritonX100 and blocked in PBS+10% FBS.
Dilution for IF 2 ug/ml
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