Parvalbumin, Synaptic Systems, 195 011C2
What do reviewers generally think about 195 011C2 from Synaptic Systems?
Researchers report that this Oyster-488-labeled parvalbumin antibody works well for single fluorescent staining of PV interneurons, with specific nuclear-associated signal and suitable 1:200 and 1:600 dilutions. It also performed on cryosections up to 150 um with high specificity and low background at 1:200. One reviewer points out that simultaneous double staining with a GABA antibody was unsuccessful because the GABA antibody lost specificity, although single stainings remained specific.
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User Reviews (3)
Review of: Parvalbumin, Synaptic Systems, 195 011C2 by Mohammad Baragji, University of Copenhagen
Single fluorescent staining targeting Parvalbumine expressing interneurons.
Primary antibody: Mouse Mab Clone 58E1, OYSTER-488-LABELED [Synaptic Systems, cat: 195 011C2], 1:200 and 1:600 dilution.
Protocol in short:
Sections were washed (3x10min), treated with target retrieval solution and washed again (1×10 min) Sections were then rinsed in FAB blocking buffer with Donkey anti-mouse followed by incubation with a fluorescent 488-labeled antibody directed towards PV (SYNAPTIC SYSTEMS) for 5 days. Sections were then washed (3×10 min) and (1×10 min) in DAPI and mounted on slides, air-dried and then mounted with cover slips.
Results:
Staining appears specific, as PV staining is intense around cell nuclei stained with DAPI. The PV antibody is thus appropriate for staining PV interneurons. The staining results further shows that both the 1:200 and 1:600 concentration are suitable.
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0 not usefulReview of: Parvalbumin, Synaptic Systems, 195 011C2 by Mohammad Baragji, University of Copenhagen
Double staining targeting Parvalbumine expressing interneurons.
Primary antibody: Mouse Mab Clone 58E1, OYSTER-488-LABELED [Synaptic Systems, cat: 195 011C2]
Secondary antibody: Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 568 conjugate [Life technologies Cat: A10042]
Protocol in short:
In short, sections were washed (3x10min), treated with target retrieval solution and washed again (1×10 min). Sections were then rinsed in FAB blocking buffer with Donkey anti-mouse followed by incubation with a rabbit antibody directed towards GABA for 2 days. Sections were then washed (3×10 min) and incubated for 4 hours with a secondary antibody, Donkey-anti-Rabbit with a fluorescent 568-labeled directed towards the primary GABA antibody. The second primary antibody 488-labeled directed towards PV was added and sections were incubated for 5 days. Sections were then washed (4×15 min) and (1×10 min) in DAPI. Finally, the sections were mounted on slides, air-dried and then mounted with cover slips.
Results:
The double staining results show that the double staining with both antibodies at the same time was not successful. The single stainings appear to be specific, but when both antibodies are combined, the GABA antibody loses its specificity.
Comments
0 useful 0 not usefulReview of: Parvalbumin, Synaptic Systems, 195 011C2 by Simon Mølgaard, Mayo Clinic
This antibody is primary labelled to oyster 488. It has been used on cryo-sections with a thickness up to 150 um with great succes. This antibody shows high specificity with very little background.
I used this at a concentration of 1:200.
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