Heparin/Heparan Sulfate, merckmillipore, MAB2040
What do reviewers generally think about MAB2040 from Sigma-Aldrich?
Researchers report mixed results with MAB2040 on HepG2 lysates at 1:1500. One reviewer found no signal by direct Western blot, while another detected a single ~50 kDa band after immunoprecipitation, using the antibody for both IP and WB. One reviewer points out that the band identity was not confirmed by another antibody and no band appeared in the input lysate control, suggesting limited confidence in specificity. Overall, performance appears inconsistent and application-dependent.
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User Reviews (2)
Review of: Heparin/Heparan Sulfate, merckmillipore, MAB2040 by Camilla G
I used this antibody (1:1500) for WB of HepG2 cell lysate, but no signal was detected
Review of: Heparin/Heparan Sulfate, merckmillipore, MAB2040 by Camilla Gustafsen, Aarhus University
We have used this antibody on HepG2 lysate.
A single band of approx. 50 kDa was detected by WB (1:1500) after IP. MAB2040 was used for both IP and WB. The identity of the band has not been confirmed by another antibody, and no band was detectable in the input lysate control.
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