Rab7A, St John's Laboratory, STJ25267
What do reviewers generally think about STJ25267 from St John's Laboratory?
Researchers report that STJ25267 performs well for immunocytochemistry and immunofluorescence, especially in primary neurons and mouse b.end3 cells, using a 1:100 dilution. One reviewer points out that it may work at lower concentrations and that human SHSY-5Y and HEK293 cells gave positive but weaker staining after PFA fixation, 0.25% saponin permeabilization, and 1 hour room-temperature staining. No major complaints are mentioned.
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User Reviews (2)
Review of: Rab7A, St John's Laboratory, STJ25267 by Marie-Louise Holm
This antibody works very well for immunocytochemistry on primary neurons.
Dilution; 1:100.
0 useful
0 not usefulReview of: Rab7A, St John's Laboratory, STJ25267 by Morten S. Nielsen, Aarhus University
Rabbit RAB7A (STJ25267) is a world-class antibody for immunofluorescence in mouse b.end3 cells!! I used it in a 1:100 dilution, but the antibody will most likely be useful in lower concentrations. The cells is PFA fixed and permabilized with 0.25 % saponin. Stained at RT for 1 hour.
Human SHSY-5Y and HEK293 cells also gave a positive, but less string signal.
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