MAP2, merckmillipore, AB5622
What do reviewers generally think about AB5622 from Sigma-Aldrich?
Researchers report MAP2, merckmillipore, AB5622 works very well for immunostaining mouse hippocampal neurons, including cultured primary hippocampal neurons, using 1:1000 dilution after 20 min 4% PFA fixation, PBS with 0.1% Triton X-100 permeabilization, and blocking. One reviewer points out that the antibody was incubated at 1:1000 in blocking buffer after permeabilization. No performance complaints are mentioned.
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User Reviews (2)
Review of: MAP2, merckmillipore, AB5622 by Anders Dalby
Worked very well for immunostaining mouse hippocampal neurons.
Neurons were fixed for 20 min in 4% PFA and permeabilized using PBS + 0.1% Triton X-100. After blocking, the neurons were incubated with antibody in a 1:1000 dilution in blocking buffer.
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0 not usefulReview of: MAP2, merckmillipore, AB5622 by Simon Mølgaard, Aarhus University
We have tested this on cultured primary hippomcampal neurons and this works very well for staining against MAP2.
We used this at a concentration of 1:1000.
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