IL-6, R&D Systems, AF-406-NA

R&D Systems AF-406-NA · IL-6 · pAb · Goat
Applications: Western Blot, Immunostaining
2.80
(Overall rating from 2 reviews) #8 of 12 targeting IL-6
Overall Rating
2.80
From 2 reviews · #8 of 12 for IL-6

Information about this antibody

Target
IL-6
Alt. Targets
Interleukin-6
Vendor
R&D Systems
Catalog No
AF-406-NA
Application
Western Blot, Immunostaining
Clonality
pAb
Origin
Goat
Reactivity
Mouse
Conjugation
N/A
Product Link
N/A

Vendor notes

No vendor notes available for this antibody.

pAbmAbs Average Ratings

ℹ
Western Blot
2.91
Overall
2.80

User Reviews (2)

Review of: IL-6, R&D Systems, AF-406-NA by Mads Kjolby, Aarhus University

Reviewed: February 19, 2014

Goat anti murine IL6 (AF406NA) from R&D works in WB for detection of murine IL6 from primary murine macrophages. I have used for subcelluar fractionation and is able to detect minutevamounts of protein. Dilute 1:1000.

Ratings from this reviewer
Western Blot

Comments

Mads KjolbyAarhus University37 reviews
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Review of: IL-6, R&D Systems, AF-406-NA by Mads Kjolby, Aarhus University

Reviewed: February 19, 2014

The IL6 antibody AF406NA against murine IL6 is less good. I have tried it in macrophages (murine) stimulated with LPS. They do express IL6 (determined by ELISA) but the western blot displays many unspecific bands, and only a possible correct one (weak) at 24 kDa. It is hard to distinguish stimulated (have IL6) from unstimulated (no IL6) cells.

WB: 1:1000

Ratings from this reviewer
Western Blot

Comments

CJS API USER7 reviews
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