IL-6, R&D Systems, AF-406-NA
What do reviewers generally think about AF-406-NA from R&D Systems?
Reviews give a mixed consensus for this goat anti-murine IL-6 antibody in Western blotting. One reviewer points out that it detects minute amounts of murine IL-6 from primary macrophages and works in subcellular fractionation at 1:1000. However, another report says it is less good in LPS-stimulated macrophages, producing many unspecific bands and only a weak possible correct band at 24 kDa, making stimulated and unstimulated samples hard to distinguish.
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User Reviews (2)
Review of: IL-6, R&D Systems, AF-406-NA by Mads Kjolby, Aarhus University
Goat anti murine IL6 (AF406NA) from R&D works in WB for detection of murine IL6 from primary murine macrophages. I have used for subcelluar fractionation and is able to detect minutevamounts of protein. Dilute 1:1000.
0 useful
0 not usefulReview of: IL-6, R&D Systems, AF-406-NA by Mads Kjolby, Aarhus University
The IL6 antibody AF406NA against murine IL6 is less good. I have tried it in macrophages (murine) stimulated with LPS. They do express IL6 (determined by ELISA) but the western blot displays many unspecific bands, and only a possible correct one (weak) at 24 kDa. It is hard to distinguish stimulated (have IL6) from unstimulated (no IL6) cells.
WB: 1:1000
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