Vimentin, merckmillipore, AB1620
What do reviewers generally think about AB1620 from Sigma-Aldrich?
The reviews indicate that the antibody performs well for immunofluorescence and Western blotting on RN22 cells. For immunofluorescence, it was used after 4% PFA fixation and 0.5% Triton X-100 permeabilization, with a best dilution of 1:40. For Western blotting, a 1:100 dilution produced a perfect Vimentin 57 kDa band. One reviewer points out that the immunofluorescence protocol used 5 min fixation and 7 min permeabilization at 4 C. No complaints are mentioned.
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User Reviews (2)
Review of: Vimentin, merckmillipore, AB1620 by Ann-Kathrin Reuschlein, Aarhus University
Used for Immunofluorescence of transfected RN22 cells treated with 4% PFA in PBS for 5 min. Washed 10 min in PBS. And penetrated with 0.5 % Triton x-100 in PBS for 7 min at 4 C.
Best dilution was 1:40.
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0 not usefulReview of: Vimentin, merckmillipore, AB1620 by Ann-Kathrin Reuschlein, Aarhus University
Used for Western blotting of RN22 cells in a dilution of 1:100
Gives a perfect Vimentin 57 kDa band.
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