Nestin, R&D Systems, AF2736
What do reviewers generally think about AF2736 from R&D Systems?
Researchers report that Nestin AF2736 performs well for immunofluorescence and Western blotting on RN22 cells. For immunofluorescence, the best dilution was 1:40 after brief PFA fixation and Triton X-100 permeabilization, producing a very strong signal. One reviewer points out that further dilutions may be needed for co-immunofluorescence to reduce signal. For Western blotting, a 1:1000 dilution detected two Nestin bands at approximately 250 and 220 kDa. No complaints were mentioned.
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Review of: Nestin, R&D Systems, AF2736 by Ann-Kathrin Reuschlein, Aarhus University
Used for Immunofluorescence of transfected RN22 cells treated with 4% PFA in PBS for 5 min. Washed 10 min in PBS. And penetrated with 0.5 % Triton x-100 in PBS for 7 min at 4 C.
Best dilution was 1:40.
Very strong signal. Try further dilutions for lesser signal, for Co-IF.
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0 not usefulReview of: Nestin, R&D Systems, AF2736 by Ann-Kathrin Reuschlein, Aarhus University
Used for Western blotting of RN22 cells in a dilution 1:1000, gives two Nestin bands at approximate 250 and 220 kDa.
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