HA, Sigma-Aldrich, 11867423001
What do reviewers generally think about 11867423001 from Sigma-Aldrich?
Researchers consistently report that this HA antibody performs well for western blot, immunoprecipitation, immunofluorescence, and immunocytochemistry, including fly testis, brain, eyes, whole mount tissue, chromosome spreads, and frozen sections. Suggested dilutions vary by application, with 1:50 to 1:100 for ICC and 1:2000 for WB, IP, or IF. One reviewer points out that it recognizes the HA peptide sequence YPYDVPDYA, is a rat IgG1 subtype, and is best for detecting HA-tagged proteins in western blots.
Information about this antibody
Vendor notes
User Reviews (3)
Review of: HA, Sigma-Aldrich, 11867423001 by Bing Zhang initiative
Works great for ICC on fly testis and brain at the dilution of 1:100. Works great for ICC on fly eyes at the dilution of 1:50.
Great on whole mount tissue and chromosome spread.
0 useful
0 not usefulReview of: HA, Sigma-Aldrich, 11867423001 by Jeannette Nardelli, Université de Paris, NeuroDiderot
Great for WB.
Great for IP.
Great for IF on frozen sections. Dilution: 1:2000
For more information see Journiac et al. Cell Reports 2020, “ Cell Metabolic Alterations due to Mcph1 Mutation in Microcephaly”.
https://www.sciencedirect.com/science/article/pii/S221112472030396X
Comments
0 useful 0 not usefulReview of: HA, Sigma-Aldrich, 11867423001 by Jon E. Silver, Arbor Vita
Anti-HA High Affinity clone 3F10 from Roche Prod. # 11867423001. The antibody recognizes the HA peptide sequence (YPYDVPDYA) derived from the influenza hemagglutinin protein.
Subtype: Rat IgG1. Best for detecting HA tagged proteins in western blots.
Comments
0 useful 0 not useful
Comments