SNX1, Everest Biotech, EB09792

Was tested for Immunofluorescence in a concentration of 1µg/ml on HEK293 and SHSY5Y cells.
Weak signal in cytoplasm and nucleus was observed, but SNX1 should normally not be present in the nucleus. Nucleus background may be reduced with blocking. However, the antibody is still weak for fluorescent imaging.
Cells were fixed in 4 % paraformaldehyde and permeabilised with 0.25 % saponine. Primary antibody was incubated 1 hour at RT.
The picture above shows SHSY5Y cells stained for SNX1.