VPS35, Everest Biotech, EB06268
What do reviewers generally think about EB06268 from Everest Biotech?
Researchers consistently report that VPS35, Everest Biotech, EB06268 performs well for immunofluorescence in mouse, human, and porcine brain endothelial cells, HepG2, HEK293, and SHSY5Y cells. It gives strong, specific vesicular staining. One reviewer points out that it detects vesicular structures colocalizing with the retromer-associated receptor SorLA. Typical conditions use 4% PFA fixation, permeabilization with 0.1% Triton X-100 or 0.25% Triton X-100/saponin, and 1:200 dilution or 1 µg/ml.
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Vendor notes
User Reviews (3)
Review of: VPS35, Everest Biotech, EB06268 by Andrea E. Toth, Aarhus University
This antibody works excellently for immunofluorescence staining in mouse (b.End3), human (hCMEC/D3) brain endothelial cell line and in primary porcine brain endothelial cells. Dilution 1:200. Fixation was done with 4% paraformaldehyde and cells were permeabilized with 0.25% Triton X-100.
0 useful
0 not usefulReview of: VPS35, Everest Biotech, EB06268 by Camilla Gustafsen, Aarhus University
Very nice for IF using HepG2 cells. The antibody detects vesicular structures colocalizing with the retromer-associated receptor SorLA.
Cells were fixed in 4% PFA and permeabilized using 0.1% Triton X100.
Dilution 1:200
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0 useful 0 not usefulReview of: VPS35, Everest Biotech, EB06268 by Morten Nielsen, Aarhus University
Was tested for Immunofluorescence in a concentration of 1µg/ml on HEK293 and SHSY5Y cells.
Very strong and specific vesicular staining. An excellent antibody for fluorescent imaging.
Cells were fixed in 4 % paraformaldehyde and permeabilised with 0.25 % saponine. Primary antibody was incubated 1 hour at RT.
The picture above shows SHSY5Y cells stained for Vps35 using this antibody (green). Nuclei are stained using Hoechst (blue).
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