VPS35, Everest Biotech, EB06268
Information about this antibody
Vendor notes
User Reviews (3)
Review of: VPS35, Everest Biotech, EB06268 by Andrea E. Toth, Aarhus University
This antibody works excellently for immunofluorescence staining in mouse (b.End3), human (hCMEC/D3) brain endothelial cell line and in primary porcine brain endothelial cells. Dilution 1:200. Fixation was done with 4% paraformaldehyde and cells were permeabilized with 0.25% Triton X-100.
0 useful
0 not usefulReview of: VPS35, Everest Biotech, EB06268 by Camilla Gustafsen, Aarhus University
Very nice for IF using HepG2 cells. The antibody detects vesicular structures colocalizing with the retromer-associated receptor SorLA.
Cells were fixed in 4% PFA and permeabilized using 0.1% Triton X100.
Dilution 1:200
Comments
0 useful 0 not usefulReview of: VPS35, Everest Biotech, EB06268 by Morten Nielsen, Aarhus University
Was tested for Immunofluorescence in a concentration of 1µg/ml on HEK293 and SHSY5Y cells.
Very strong and specific vesicular staining. An excellent antibody for fluorescent imaging.
Cells were fixed in 4 % paraformaldehyde and permeabilised with 0.25 % saponine. Primary antibody was incubated 1 hour at RT.
The picture above shows SHSY5Y cells stained for Vps35 using this antibody (green). Nuclei are stained using Hoechst (blue).
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0 useful 0 not useful
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