AP2A1, Everest Biotech, EB11875

Everest Biotech EB11875 · AP2A1 · pAb · Goat
Applications: Western Blot, ELISA
3.17
(Overall rating from 1 review)
#1 of 1 targeting AP2A1
3.17
From 1 review · #1 of 1 for AP2A1

Information about this antibody

Target
Alt. Targets
AP2A1
Catalog No
EB11875
Application
Clonality
pAb
Origin
Goat
Reactivity
Human
Conjugation
N/A
Product Link
N/A

Vendor notes

No vendor notes available for this antibody.

pAbmAbs Average Ratings

ℹ
Immunostaining
3.18
Overall
3.17

User Reviews (1)

Review of: AP2A1, Everest Biotech, EB11875 by Morten Nielsen, Aarhus University

Reviewed: October 25, 2013

Was tested for Immunofluorescence in a concentration of 1µg/ml on HEK293 and SHSY5Y cells.
Weak signal in cytoplasm and nucleus was observed, but AP2A1 should normally not be present in the nucleus. Nucleus background may be reduced with blocking. However, the antibody is still weak for fluorescent imaging.
Cells were fixed in 4 % paraformaldehyde and permeabilised with 0.25 % saponine. Primary antibody was incubated 1 hour at RT.
The picture above shows SHSY5Y cells stained for AP2A1 using this antibody (green). Nuclei are stained using Hoechst (blue).

Immunostaining

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