HSPA8, Everest Biotech, EB07295
What do reviewers generally think about EB07295 from Everest Biotech?
Researchers report that HSPA8, Everest Biotech, EB07295 performed poorly in immunofluorescence and Western blot. One reviewer found no clear signal in primary hippocampal neurons at 0.5, 1, or 2 ug/mL and no detection at 1:5000 in WB. One reviewer points out that weak cytoplasmic and nuclear signal was observed in HEK293 and SHSY5Y cells at 1 ug/mL, corresponding to expected localization, but it was not optimal for fluorescent imaging. Overall, the reviews indicate it is not reliable or optimal for these applications.
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User Reviews (2)
Review of: HSPA8, Everest Biotech, EB07295 by Anders Dalby
This antibody did not work for me in both immunostaining and WB.
IF: Primary hippocampal neruons from mice were fixed for 20 min in 4% PFA and permeabilized using PBS + 0.1% Triton X-100. After blocking, the neurons were incubated with antibody in a both 0,5ug/mL, 1ug/mL and 2ug/mL dilution in blocking buffer. No clear signal was observed using these concentrations.
WB: I used 1:5000 dilution of primary hippocampal neurons and was unable to detect HSPA8.
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0 not usefulReview of: HSPA8, Everest Biotech, EB07295 by Morten Nielsen, Aarhus University
Was tested for Immunofluorescence in a concentration of 1µg/ml on HEK293 and SHSY5Y cells.
Weak signal in cytoplasm and nucleus was observed, corresponding to expected localisation. However, not optimal for fluorescent imaging.
Cells were fixed in 4 % paraformaldehyde and permeabilised with 0.25 % saponine. Primary antibody was incubated 1 hour at RT.
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