HSPA8, Everest Biotech, EB07295
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Review of: HSPA8, Everest Biotech, EB07295 by Anders Dalby
This antibody did not work for me in both immunostaining and WB.
IF: Primary hippocampal neruons from mice were fixed for 20 min in 4% PFA and permeabilized using PBS + 0.1% Triton X-100. After blocking, the neurons were incubated with antibody in a both 0,5ug/mL, 1ug/mL and 2ug/mL dilution in blocking buffer. No clear signal was observed using these concentrations.
WB: I used 1:5000 dilution of primary hippocampal neurons and was unable to detect HSPA8.
0 useful
0 not usefulReview of: HSPA8, Everest Biotech, EB07295 by Morten Nielsen, Aarhus University
Was tested for Immunofluorescence in a concentration of 1µg/ml on HEK293 and SHSY5Y cells.
Weak signal in cytoplasm and nucleus was observed, corresponding to expected localisation. However, not optimal for fluorescent imaging.
Cells were fixed in 4 % paraformaldehyde and permeabilised with 0.25 % saponine. Primary antibody was incubated 1 hour at RT.
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