NCAM, merckmillipore, MAB5324

Antibodies directed against the polysialylated form of the neural cell-adhesion molecule (PSA-NCAM) are appropriate for the detection of brain regions with enhanced plasticity. Therefore, Merck Millipore (formerly Chemicon) offers the recommendable MAB5324 (an IgM, clone 2-2B). This marker was successfully applied to tissue sections from hibernating ground squirrels based on immunoperoxidase staining with nickel-enhanced diaminobenzidine as chromogen (Arendt et al 2003 J Neurosci – Fig 8). Moreover, PSA-NCAM was recently revealed by indirect immunofluorescence labelling with µ-chain specific secondary antibodies, which recognize IgMs but no IgGs (Mack et al 2018 Brain Struct Funct – Fig 5).

I highly recommend this antibody for IHC

Arendt et al. 2003 J Neurosci: http://www.jneurosci.org/content/23/18/6972.long
Mack et al. 2018 Brain Struct Funct: https://link.springer.com/article/10.1007%2Fs00429-018-1730-1