Phospho-Akt (Thr308), Cell Signaling Technology, 2965
What do reviewers generally think about 2965 from Cell Signaling Technology?
Researchers report the antibody performs well for detecting phospho-AKT in cultured cells, using a 1:800 dilution. It detected AKT phosphorylation in hippocampal neurons after 1 nM BDNF stimulation for 10 minutes, and in 293 cells after insulin stimulation, with increased staining at 1 and 10 nM versus unstimulated controls. One reviewer points out that the 1:800 dilution worked well in their neuronal BDNF assay.
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Review of: Phospho-Akt (Thr308), Cell Signaling Technology, 2965 by Simon Mølgaard
I have also tested this on cultured hippocampal neurons stimulated with 1 nM of BDNF for 10 minutes. This works great to detect phosphorylation of AKT.
Dilution 1:800
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0 not usefulReview of: Phospho-Akt (Thr308), Cell Signaling Technology, 2965 by Simon Jensen
This antibody was tested on 293 cells in culture. The cells were stimulated with either 0, 1, 10 or 100nM of insulin before being fixed and stained. This antibody showed increased staining af cells stimulated with 1 and 10nM, compared to unstimulated.
Dilution 1:800
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