Phospho-Akt (Ser473), Cell Signaling Technology, 4058
What do reviewers generally think about 4058 from Cell Signaling Technology?
Researchers report that Phospho-Akt (Ser473) CST 4058 can detect increased phospho-Akt in cultured cells after stimulation, but the signal is consistently very weak. One reviewer points out that in cultured hippocampal neurons stimulated with 1 nM BDNF for 10 minutes, the antibody may detect increased phosphorylation but the signal is too weak, so they recommend using other antibodies. Another reviewer found increased staining in 293 cells stimulated with 1 and 10 nM insulin versus unstimulated, yet staining remained very weak at all insulin concentrations. Both used 1:200 dilution.
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Review of: Phospho-Akt (Ser473), Cell Signaling Technology, 4058 by Simon Mølgaard
I have also tested this on cultured hippocampal neurons stimulated with 1 nM of BDNF for 10 minutes. Although this might detect increased phosphorylation of AKT the signal is very weak and i recommend using other antibodies for this.
Dilution 1:200
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0 not usefulReview of: Phospho-Akt (Ser473), Cell Signaling Technology, 4058 by Simon Jensen
This antibody was tested on 293 cells in culture. The cells were stimulated with either 0, 1, 10 or 100nM of insulin before being fixed and stained. This antibody showed increased staining af cells stimulated with 1 and 10nM, compared to unstimulated, however the staining was very weak at all concentrations.
Dilution 1:200
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