Phospho-Akt (Ser473), Cell Signaling Technology, 4058
Information about this antibody
Target
Alt. Targets
Catalog No
4058
Application
Clonality
mAb
Origin
Rabbit
Reactivity
Human, Mouse, Rat
Conjugation
N/A
Product Link
N/A
Vendor notes
No vendor notes available for this antibody.
User Reviews (2)
Review of: Phospho-Akt (Ser473), Cell Signaling Technology, 4058 by Simon Mølgaard
I have also tested this on cultured hippocampal neurons stimulated with 1 nM of BDNF for 10 minutes. Although this might detect increased phosphorylation of AKT the signal is very weak and i recommend using other antibodies for this.
Dilution 1:200
0 useful
0 not usefulReview of: Phospho-Akt (Ser473), Cell Signaling Technology, 4058 by Simon Jensen
This antibody was tested on 293 cells in culture. The cells were stimulated with either 0, 1, 10 or 100nM of insulin before being fixed and stained. This antibody showed increased staining af cells stimulated with 1 and 10nM, compared to unstimulated, however the staining was very weak at all concentrations.
Dilution 1:200
Comments
0 useful 0 not useful
Comments