phospho-Mapk (p44/42), Cell Signaling Technology, 4377
What do reviewers generally think about 4377 from Cell Signaling Technology?
Researchers report poor performance of this phospho-Mapk antibody in stimulation experiments. One reviewer points out that at 1:200 dilution in 293 cells stimulated with 0, 1, 10, or 100 nM insulin, it showed almost no difference between stimulated and unstimulated samples. Another reviewer found very limited success in cultured hippocampal neurons stimulated with 1 nM BDNF for 10 minutes. Overall, the reviews suggest limited reliability for detecting stimulation-induced phospho-Mapk in these cell systems.
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Review of: phospho-Mapk (p44/42), Cell Signaling Technology, 4377 by Simon Mølgaard
I have also tested this antibody on cultured hippocampal neurons stimulated with 1 nM of BDNF for 10 minutes with very limited succes.
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0 not usefulReview of: phospho-Mapk (p44/42), Cell Signaling Technology, 4377 by Simon Jensen
This antibody was tested on 293 cells in culture. The cells were stimulated with either 0, 1, 10 or 100nM of insulin before being fixed and stained.This antibody showed almost no difference between stimulated and unstimulated.
Dilution 1:200
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