SorCS1, Academia, Academia-0013
What do reviewers generally think about Academia-0013 from Academia?
Researchers generally report that SorCS1, Academia, Academia-0013 performs well for detecting human and mouse SorCS1, especially in Western blots of HeLa lysates at 1:2500 and as a capture antibody in a homemade ELISA at 10 ug/ml. It also stains stable CHO cells expressing mouse SorCS1 variant c, with untransfected cells unstained at 3 ug/ml. One reviewer points out that the antibody appears old, possibly homemade, and stored at 4 degrees, and that the CHO cell membrane staining was weak and dotted. Another asks where to obtain it.
User Reviews (4)
Review of: SorCS1, Academia, Academia-0013 by Melkam Kebede, University of Sydney
Where can one get this antibody? Really interested in trying it in our cells?
Thanks,
0 useful
0 not usefulReview of: SorCS1, Academia, Academia-0013 by Peter Lund Ovesen, University of Aarhus
This antibody (9,6 ug/ul) works excellent for detecting human and mouse SorCS1 in lysates of HeLa cells overexpressing the protein.
Western blot Dilution 1:2500
Comments
0 useful 0 not usefulReview of: SorCS1, Academia, Academia-0013 by Mads Kjolby, University of Aarhus
This antibody works great in a home-made ELISA as capture antibody for detection of human SorCS1.
I have used 10 ug/ml when coating the maxisorp plate (55 mM sodium bicarbonate, pH 9.8).
Details can be acquired upon request.
Comments
0 useful 0 not usefulReview of: SorCS1, Academia, Academia-0013 by Jenny Blechingberg Friis, University of Aarhus
This antibody is an old antibody (homemade??) and stored in 4 degrees. It was used to stain CHO-cells stable expressing mouse SorCS1 variant c. The membrane of CHO/mSorCS1c stain weakly in a dotted pattern and the untransfected CHO-cells are without staining at 3 ug/ml.
Comments
0 useful 0 not useful
Comments