LDLR, Abcam, ab60107
What do reviewers generally think about ab60107 from Abcam?
Researchers report that ab60107 works well for immunofluorescence of endogenous LDLR in HepG2 cells, with intense staining at 1:100 and expected weak signal in PCSK9-overexpressing cells. It failed for Western blot at 1:1000 in HepG2 lysate and for immunoprecipitation of endogenous LDLR from HEK293 cells, giving many nonspecific bands and pulling down SorLA. One reviewer points out that use of 10 µg/ml works very well for IF staining in HepG2 cells.
Information about this antibody
Vendor notes
User Reviews (4)
Review of: LDLR, Abcam, ab60107 by Camilla Gustafsen, Aarhus University
This antibody (1:1000) does not work for WB detection of endogenous LDLR in HepG2 cell lysate.
0 useful
0 not usefulReview of: LDLR, Abcam, ab60107 by Laura Tvilling Poulsen (Aarhus University)
Anti-LDLR, ab60107, mouse.
Using this antibody (1:100) we saw an intense IF staining of endogenous LDLR in HepG2 cells. As control we used HepG2 cells with overexpression of PCSK9, and the antibody signal in these cells was, as expected, very weak.
Comments
0 useful 0 not usefulReview of: LDLR, Abcam, ab60107 by Simon Glerup, Aarhus University
This antibody does not work for IP of endogenous LDL receptor from HEK293 cells.
We did IPs from metabolically labelled cells (S35)and saw a lot of unspecific bands. The antibody even pulled down the related receptor SorLA.
Comments
0 useful 0 not usefulReview of: LDLR, Abcam, ab60107 by Camilla Gustafsen
This antibody works very well for IF staining of LDLR in HepG2 cells. Use 10 µg/ml.
Comments
0 useful 0 not useful
Comments