Cholera toxin B, List Biological Labs, 703
What do reviewers generally think about 703 from List Biological Labs?
Researchers generally report that Cholera toxin B, List Biological Labs, 703 performs well for detecting bound or internalized toxin in fixed cells and tissues, including perfusion-fixed mouse tissue, 293 cells, and hippocampal neurons, at dilutions of 1:1000 to 1:5000. One reviewer points out that it was not very useful for detecting retrogradely transported toxin on PFA-fixed mouse brain cryosections because of strong cross-reaction with blood vessels.
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User Reviews (3)
Review of: Cholera toxin B, List Biological Labs, 703 by David I. Hughes, University of Glasgow
This antibody worked great for immunostaining of perfusion-fixed mice tissue for fluorescence microscopy.
Dilution used: 1:5000
For more information see Boyle et al., Defining a Spinal Microcircuit that Gates Myelinated Afferent Input: Implications for Tactile Allodynia, Cell Reports, vol 28, issue 2, p526-540, July 9, 2019 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30806-X).
Hughes Laboratory:
https://specciebear.wixsite.com/spinal-cord-hughes
0 useful
0 not usefulReview of: Cholera toxin B, List Biological Labs, 703 by Simon Glerup, Aarhus
Excellent antibody for staining cholera toxin subunit b bound and internalized by 293 cells and hipppcampal neurons.
Dilution 1:1000
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0 useful 0 not usefulReview of: Cholera toxin B, List Biological Labs, 703 by Simon Glerup
We used this cholera toxin B antibody at dilution 1:1000 for the detection retrogradely transported cholera toxin B subunit on cryosections of PFA-fixed mouse brain. The antibody was not very useful. There was strong cross reaction with blood vessels.
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