SorLA, R&D Systems, AF5699
What do reviewers generally think about AF5699 from R&D Systems?
Researchers generally report that SorLA, R&D Systems, AF5699 performs well for immunostaining and IHC, including ICC on transiently transfected HepG2 cells at 1:1000, immunostaining of adult mouse brain tissue and cultured hippocampal neurons at 5 ug/ml, and IHC on PFA-fixed mouse brain cryosections with antigen retrieval, where knockout sections were blank. One reviewer points out that it did not work very well on rat hippocampal neurons, producing substantial background under their PFA, sucrose, Triton X-100, and gelatin protocol.
Information about this antibody
Vendor notes
User Reviews (4)
Review of: SorLA, R&D Systems, AF5699 by Julie Torvund-Jensen
Works very well for ICC on HepG2 cells transiently transfected with full-length human SorLA.
Dilution 1:1000.
0 useful
0 not usefulReview of: SorLA, R&D Systems, AF5699 by Stine Klinger, NIH, Washington
I have tested this antibody on rat hippocampal neurons. It doesn’t work very well and gives a lot of background.
Protocol details:
Fixed with 4% PFA, 4% sucrose.
Permeabilized with 0.02% Triton X-100 in gelatin
Blocked with gelatin
Antibody incubation: in gelatine, 30 min 37 degrees.
The image above shows an example of a hippocampal neuron stained for MAP2(blue) and SorLA using AF5699 (red).
Comments
0 useful 0 not usefulReview of: SorLA, R&D Systems, AF5699 by Simon Mølgaard, Aarhus University
I have used this antibody for immunostainings on adult mouse barin tissue as well as cultured hipoocampal neurons. In both cases this antibody worked very well for detection of SorLA.
concentration 5ug/ml
Comments
0 useful 0 not usefulReview of: SorLA, R&D Systems, AF5699 by Simon Glerup, Aarhus University
We have used this SorLA antibody for IHC on PFA-fixed cryosections of mouse brain treated with antigen retrieval kit.
The antibody is great and sections from SorLA knockouts are blank confirming its specificity.
Dilution: 5 ug/ml
Comments
0 useful 0 not useful
Comments