SorLA, R&D Systems, AF5699

R&D Systems AF5699 · SorLA · pAb · Sheep
Applications: Western Blot, Immunostaining
4.05
(Overall rating from 4 reviews) #6 of 15 targeting SorLA
Overall Rating
4.05
From 4 reviews · #6 of 15 for SorLA

Information about this antibody

Target
SorLA
Alt. Targets
LR11
Vendor
R&D Systems
Catalog No
AF5699
Application
Western Blot, Immunostaining
Clonality
pAb
Origin
Sheep
Reactivity
Human, Mouse
Conjugation
N/A
Product Link
N/A

Vendor notes

No vendor notes available for this antibody.

pAbmAbs Average Ratings

ℹ
Immunostaining
4.05
Overall
4.05

User Reviews (4)

Review of: SorLA, R&D Systems, AF5699 by Julie Torvund-Jensen

Reviewed: January 23, 2018

Works very well for ICC on HepG2 cells transiently transfected with full-length human SorLA.
Dilution 1:1000.

Ratings from this reviewer
Immunostaining

Comments

Julie Torvund-JensenDepartment of Biomedicine, Aarhus University14 reviews
Thumbs up 0 useful
Thumbs down 0 not useful

Review of: SorLA, R&D Systems, AF5699 by Stine Klinger, NIH, Washington

Reviewed: March 19, 2014

I have tested this antibody on rat hippocampal neurons. It doesn’t work very well and gives a lot of background.
Protocol details:
Fixed with 4% PFA, 4% sucrose.
Permeabilized with 0.02% Triton X-100 in gelatin
Blocked with gelatin
Antibody incubation: in gelatine, 30 min 37 degrees.
The image above shows an example of a hippocampal neuron stained for MAP2(blue) and SorLA using AF5699 (red).

Ratings from this reviewer
Immunostaining

Comments

Stine KlingerNIH, Washington9 reviews
Thumbs up 0 useful
Thumbs down 0 not useful

Review of: SorLA, R&D Systems, AF5699 by Simon Mølgaard, Aarhus University

Reviewed: January 29, 2014

I have used this antibody for immunostainings on adult mouse barin tissue as well as cultured hipoocampal neurons. In both cases this antibody worked very well for detection of SorLA.

concentration 5ug/ml

Ratings from this reviewer
Immunostaining

Comments

Simon MølgaardAarhus University105 reviews
Thumbs up 0 useful
Thumbs down 0 not useful

Review of: SorLA, R&D Systems, AF5699 by Simon Glerup, Aarhus University

Reviewed: June 24, 2012

We have used this SorLA antibody for IHC on PFA-fixed cryosections of mouse brain treated with antigen retrieval kit.

The antibody is great and sections from SorLA knockouts are blank confirming its specificity.

Dilution: 5 ug/ml

Ratings from this reviewer
Immunostaining

Comments

Simon GlerupAarhus University238 reviews
Thumbs up 0 useful
Thumbs down 0 not useful

Submit Your Review of SorLA, R&D Systems, AF5699

Please log in to submit a review.