Calretinin, Sigma-Aldrich, AB5054
What do reviewers generally think about AB5054 from Sigma-Aldrich?
Researchers report that Calretinin, Sigma-Aldrich, AB5054 performs well in IHC on mouse cryosections, including embryonic tissue, olfactory bulb, and spinal cord, and in hippocampal lysates, giving specific, intense nuclear and cytoplasmic staining with low background. It works with DAB and fluorescence at dilutions of 1:250 to 1:1000. One reviewer points out that cultured hippocampal neurons at 1:1000 show clear positive/negative distinction but slightly higher background, and that IP/WB were not tested.
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User Reviews (5)
Review of: Calretinin, Sigma-Aldrich, AB5054 by Simon Boggild
I have used this antibody on cryosections of mouse embryonic tissue. It stains very well, clearly delineating cells known to express calretinin. Very high signal to background contrast. Dilution used were 1:250 but 1:500 also works well.
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0 not usefulReview of: Calretinin, Sigma-Aldrich, AB5054 by Hande Login
I performed IHC with this antibody on 12 um cryosections of mouse olfactory bulb. I got very nice, specific staining of interneurons without any background.
Antibody dilution: 1:500
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0 useful 0 not usefulReview of: Calretinin, Sigma-Aldrich, AB5054 by Simon Mølgaard, Aarhus University
This was tested os hippocampal lysates from p20 mice.This showed a very nice staining with very litttle background. Used at 1:1000
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0 useful 0 not usefulReview of: Calretinin, Sigma-Aldrich, AB5054 by Maj Ulrichsen
I have used this antibody on spinal cord cryo-sections at a dilution of 1:500. It is an excellent antibody that shows intense staining of a dense band of cells in the superficial lamina and of some cells in the deeper laminae of the dorsal horn. It shows staining both in the nuclei and the cytoplasm of positive cells and has a low background staining.
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0 useful 0 not usefulReview of: Calretinin, Sigma-Aldrich, AB5054 by Simon Jensen, Aarhus
This antibody works very well for staining of tissue, both using DAB and fluorescence. At a concencration of 1:1000 very low background is seen.
Staining of hippocampal neurons in culture using a concentration of 1:1000 also showed a clear distinction between calretinin-positive and -negative cells. However, in this case the background is a little bit higher.
I have not tested it for IP or WB.
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