Calbindin, millipore, AB1778
What do reviewers generally think about AB1778 from Sigma-Aldrich?
Researchers report that Calbindin, millipore, AB1778 performs well in immunofluorescence and IHC on mouse cryosections, including embryonic tissue, olfactory bulb, and spinal cord, giving strong, specific staining with minimal or low background. Useful dilutions range from 1:50 to 1:2000, with DAB at 1:2000 and fluorescence at 1:1000 for tissue and cultured neurons. One reviewer points out that the antibody was not tested for IP or WB.
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User Reviews (4)
Review of: Calbindin, millipore, AB1778 by Simon Boggild
I have used this antibody on 10 µm cryosections of mouse embryonic tissue for immunofluorescence. I observed very strong specific staining both in nervous and peripheral tissues with minimal background even though using a concentrated dilution.
Dilution 1:50
0 useful
0 not usefulReview of: Calbindin, millipore, AB1778 by Hande Login
I performed IHC with this antibody on 12 um cryo sections of mouse olfactory bulb sections. I got excellent specific staining of interneurons without any background.
Antibody dilution: 1:500
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0 useful 0 not usefulReview of: Calbindin, millipore, AB1778 by Maj Ulrichsen
I have used this antibody on 20 um spinal cord cryo-sections at a dilution of 1:500. It works very well and shows low background staining along with intense cytoplasm and nuclear staining of cells.
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0 useful 0 not usefulReview of: Calbindin, millipore, AB1778 by Simon Jensen, Aarhus
DAB staining of tissue with this antibody showed high specificity and very low background at a concentration of 1:2000. For fluorescence staining of tissue. the optimal concentration was 1:1000.
This was also tested on neurons in culture where a clear difference between Calbindin-positive and -negative was seen at a concentration of 1:1000.
This was not tested for IP or WB.
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