TGN46, Bio-Rad Laboratories, AHP500

Works very nicely for IF (HEK293 and HepG2 cells).
I have used a 1:500 dilution and overnight incubation,

For IF, dilution at 1:500 to 1:1000 is working good after overnight incubation.

I used this antibody as a marker for TGN in HEK293 cells in confocal fluorescence with good results (1:100).

This antibody is very good for immunostaining of HepG2 cells. We used it 1:100, but I reckon that it can be diluted more and still give a nice result

We used this TGN46 antibody 1:100. It works fine for immunostaining of HEK293 cells.

We used it in Western blotting on sucrose gradient fractions.

It recognizes a very faint band of the correct size at the expected density. A stronger (but still weak) band is observed in the post nuclear supernatant of 293 cells.

Dilution 1:500