Golgin-84, BD Bioscience, 611382
What do reviewers generally think about 611382 from BD Bioscience?
Researchers report limited utility for Golgin-84, BD Bioscience, 611382. One reviewer points out that it cannot be used for immunolabeling of HepG2 cells at 1.25 µg antibody/ml. Another used it in Western blotting of sucrose gradient fractions at 1:1000, detecting only a very faint band of the correct size in the post nuclear supernatant of 293 cells, with no band in the fractions. Overall, the antibody shows limited performance, with failed HepG2 immunolabeling and faint Western blot detection.
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User Reviews (2)
Review of: Golgin-84, BD Bioscience, 611382 by Camilla Gustafsen
This antibody can not be used for immunolabeling of HepG2 cells. We used 1.25 µg antibody/ml
0 useful
0 not usefulReview of: Golgin-84, BD Bioscience, 611382 by Simon Glerup, Aarhus
We used it in Western blotting on sucrose gradient fractions.
It recognizes a very faint band of the correct size in the post nuclear supernatant of 293 cells. No band is observed in the fractions
Dilution 1:1000
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