P75NTR, Promega Corporation, G323A
What do reviewers generally think about G323A from Promega Corporation?
Researchers recommend P75NTR (Promega G323A) as a rabbit antiserum against an intracellular p75 epitope, useful for immunofluorescence and immunostaining. It detected p75 in forebrain cholinergic projection neurons in hamsters and is suitable for detecting p75 across various mammalian species, unlike some extracellular epitope antibodies. One reviewer points out that it works for immunostaining p75 in transfected HEK293 cells at a 1:200 dilution. No complaints are mentioned.
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User Reviews (2)
Review of: P75NTR, Promega Corporation, G323A by Wolfgang Härtig, University of Leipzig
I can highly recommend this antibody:
A rabbit antiserum raised against an intracellular epitope of the human nerve growth factor receptor p75 (Promega, Madison, WI, USA; G323A) was consistently useful to reveal this marker which is in forebrain tissues exclusively by cholinergic projection neurons. This is exemplified by immunofluorescence labelling of the basal forebrain in hamsters (Härtig et al 2007, Fig 4). Notably, this immunoreagent is appropriate for the detection of p75 in various mammalian species – in contrast to antibodies recognizing extracellular epitopes frequently only in rat or mice or human and rabbit.
Härtig et al. 2007: https://onlinelibrary.wiley.com/doi/full/10.1111/j.1460-9568.2006.05250.x
Review of: P75NTR, Promega Corporation, G323A by Anja A
This antibody works for immunostaining of p75 in transfected HEK293 cells.
Dilution 1:200
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