LAMP-1, Abcam, ab24170
What do reviewers generally think about ab24170 from Abcam?
Researchers generally report that ab24170 performs well for detecting LAMP-1 in murine primary neurons, astrocytes, and hippocampal neurons by Western blot and lysosomal staining, giving clear bands with little background at 1:1000 for WB and 1:300 for staining. It reportedly failed on HEK293 lysates, likely due to lack of human LAMP-1 cross-reactivity, though one reviewer says it stains 293 cells. One reviewer points out that saponin permeabilization gives much better lysosome staining than Triton X-100, and leupeptin/pepstatin pretreatment can improve visualization.
Information about this antibody
Vendor notes
User Reviews (6)
Review of: LAMP-1, Abcam, ab24170 by Anders Dalby
Works great for detection of LAMP-1 in primary murine neurons (cortical). A clear band with no background signal was obtained after short exposure.
Dilution 1:1000
0 useful
0 not usefulReview of: LAMP-1, Abcam, ab24170 by Trine Rasmussen
This antibody worked well for detecting LAMP1 in lysates from murine primary cortical neurons. Clear bands were detected after 30 sec. of exposure with ECl prime (Amersham).
Dilution 1:1000
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0 useful 0 not usefulReview of: LAMP-1, Abcam, ab24170 by Sérgio Almeida
This antibody works very well for the detection of LAMP1 in lysates of primary mouse neuronal cultures through WB. (Dilution of 1:1000)
It did not work for HEK293 cell lysates, probably because it does not cross-react with the human LAMP1.
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0 useful 0 not usefulReview of: LAMP-1, Abcam, ab24170 by Simon Glerup, Aarhus
This antibody stains vesicles in 293 cells that appear to be located in the nucleus or just above it.
I doubt that these are lysosomes! 293 cells were permeabilized with 0.1% TritonX100
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0 useful 0 not usefulReview of: LAMP-1, Abcam, ab24170 by Anja A, Aarhus
This antibody also works for staining of 293 cells.
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0 useful 0 not usefulReview of: LAMP-1, Abcam, ab24170 by Simon Glerup, Aarhus
We use this antibody as a lysosomal marker in mouse-derived cell lines, e.g. primary astrocytes and hippocampal neurons.
Dilution 1:300.
NB. For human cell lines, we use mAb H4A3.
It is important to consider what detergent is used for permeabilization of cells. Saponin appears to give much better lysosome stainings than Triton X-100.
For inhibition of lysosomal degradation and better visualization of lysosomes, incubate with 50 ug/ml leupeptin and pepstatin for 24 h before fixing the cells. During this period, the leupeptin/pepstatin medium has to be changed every six hours.
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