SorCS1, VWR International, cy3

I have tested both 510 and 511 in Western blot. No signal at all, even when used in 1:10 dilution.
Samples: Mouse adult brain WT and SorCS1-/-, p0 WT brain and CHO cells stable expressing either mouse or human SorCS1 variant c.

Animal 511:
This antibody was used to stain CHO cells stable expressing mouse SorCS1 variant c. It stains the membrane specifically,but background staining is also seen in untransfected control cells at 1:100 concentration. The signal/background ratio is best in the second bleed.

We had this antibody custom made by Genovac using DNA immunization of rabbits with a plasmid encoding mouse SorCS1.

Second bleed is excellent in specifically detecting mouse SorCS1 in Western blotting. No cross reactivity with human SorCS1.

It also stains primary cultures derived from wild-type mice but not from SorCS1 knockouts.