Phospho-serine, Sigma-Aldrich, AB1603
What do reviewers generally think about AB1603 from Sigma-Aldrich?
Researchers report AB1603 works well for Western blot and immunoprecipitation, detecting serine-phosphorylated proteins with clear bands. WB dilution 1:1000 is consistently used, including SY5Y cells and BDNF-stimulated TrkB-transfected cells, where a dose-dependent response and an intense ~50 kDa band were observed. One reviewer points out that 1 µg/mL was sufficient for IP of P32-labelled lysates. The reviews do not mention complaints.
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User Reviews (3)
Review of: Phospho-serine, Sigma-Aldrich, AB1603 by Anders Dalby
I have used this antibody for both WB and IP and works well in both categories.
Phosphorus-32 labelled cells were lysed and by using 1ug/mL i was able to IP and see clear distinct bands corresponding to serine-phosphorylated proteins.
For WB i use 1:1000 dilution. Works well.
0 useful
0 not usefulReview of: Phospho-serine, Sigma-Aldrich, AB1603 by Lars Meinertz Byg
I have also had good experiences with this antibody in detecting pSerin-residues in a 1:1000 dilution in SY5Y-cells
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0 useful 0 not usefulReview of: Phospho-serine, Sigma-Aldrich, AB1603 by Simon Glerup, Aarhus University
I had heard a lot of negative things about phospho-serine antibodies but this one actually seems to work.
We stimulated SY5Y neuroblastoma cells transfected with human TrkB with increasing concentrations of BDNF for 15 min. Cells were lysed in TNE buffer containing protease and phosphatase inhibitors. Lysates were analyzed by Western blotting and blots were developed using ECL Plus.
We observed a dose-dependent response to BDNF using this antibody. In particular one intense band around 50 kDa was induced by BDNF in addition to several weaker bands.
WB dilution 1:1000
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