Parvalbumin, Abcam, ab11427
What do reviewers generally think about ab11427 from Abcam?
Researchers report mixed performance for Parvalbumin, Abcam, ab11427. It works well for IHC/immunofluorescence on mouse brain and spinal cord cryosections, with clear parvalbumin-positive cells at 1:500 to 1:1000, and one reviewer found DAB staining excellent at 1:4000. Western blot results are less favorable: several reviewers describe weak bands, background, and additional bands at 1:1000 or 0.1 ug/ml. One reviewer points out that intense parvalbumin-positive cells in spinal cord were easy to distinguish from background, including soma, nucleus, and some processes.
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User Reviews (6)
Review of: Parvalbumin, Abcam, ab11427 by Abdel-Rahman Al-Absi
PARVALBUMIN RABBIT ANTI-RAT ABCAM AB11427
I have used this antibody to do western blot on mouse frozen tissue. I have incubated with this antibody (1:1000) at 4 °C over night, then with secondary antibody at room temperature for 1 hour. The antibody gives weak band.
0 useful
0 not usefulReview of: Parvalbumin, Abcam, ab11427 by Niels Degn, Aarhus University
I have used this antibody for IHC on crysections from PFA perfusion fixed mouse brains. Works very well for detection of parvalbumin positive interneurons in the striatum in a dilution of 1:500.
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0 useful 0 not usefulReview of: Parvalbumin, Abcam, ab11427 by Veerle Paternoster, Aarhus University
Tested on mouse brain cell lyste (loaded 30 ug and 10 ug) using stainfree gel/membrane, semi-dry blotting and detected with ECL signal.
A weaker band, of appropriate size, together with some more intense bands of other sizes.
used dilution 1/1000.
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0 useful 0 not usefulReview of: Parvalbumin, Abcam, ab11427 by Simon Mølgaard, Aarhus University
This was tested os hippocampal lysates from p20 mice. It gave a weak staining with some background.
used at 0.1ug/ml
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0 useful 0 not usefulReview of: Parvalbumin, Abcam, ab11427 by Maj Ulrichsen, Aarhus University
I have used this antibody on 20 um spinal cord cryo-sections. It was diluted 1:500 and detected with a fluorescent secondary antibody. Intense parvalbumin-positive cells were very easy to distinguish from background staining. They were small cells and parvalbumin-immunoreactive in the soma and the nucleus. Also, what appeared to be neuronal processes stained positive for parvalbumin in some areas of the spinal cord.
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0 useful 0 not usefulReview of: Parvalbumin, Abcam, ab11427 by Simon Jensen, Aarhus University
I used this antibody for staining of hippocampal nuerons in culture as well as staining of tissue, both with fluorescence as well as DAB staining. In all cases this antibody works excellent. For DAB staining i used it 1:4000 while it for fluorescent staining of tissue as well as cells in culture was used 1:1000
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