Oligodendrocyte marker O4, R&D Systems, mab1326
What do reviewers generally think about mab1326 from R&D Systems?
Researchers report that O4 mab1326 performs well for immunofluorescence/immunostaining of O4 in rat and murine primary oligodendrocyte cultures, using the manufacturer protocol or dilutions around 1:500 to 1:1000, with one reviewer noting it can be used at lower dilutions without problems. One reviewer points out that some background staining was observed despite overall good performance. No other major complaints are mentioned.
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User Reviews (3)
Review of: Oligodendrocyte marker O4, R&D Systems, mab1326 by Nicola Micali, Yale University
Great antibody for IF. Concentration indicated by manufacturer’s protocol.
For more information see Micali et al. Cell Reports 2020, “Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates”.
https://www.sciencedirect.com/science/article/pii/S2211124720305489
0 useful
0 not usefulReview of: Oligodendrocyte marker O4, R&D Systems, mab1326 by Julie Torvund-Jensen, Aarhus University
I have used this antibody for immunstaining of O4 in rat oligodendrocytes. The ab works nicely, however, some background staining has been observed. Dilution 1:1000
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0 useful 0 not usefulReview of: Oligodendrocyte marker O4, R&D Systems, mab1326 by Christian Vægter
We tested this ab for IF on murine primary oligodendrocyte cultures, with great success.
Dilution 1:500, but can be more dilute without problems.
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