NMDA receptor 2B, Sigma-Aldrich, 06-600
What do reviewers generally think about 06-600 from Sigma-Aldrich?
Researchers report that the antibody performs well for Western blotting of mouse hippocampal membrane preparations, with good to excellent results at dilutions of 1:1000 and 1:5000. One reviewer points out that using a lower antibody concentration reduced unspecific binding and yielded only the NR2B band. However, another reviewer found it did not work for immunostaining cultured hippocampal neurons at 1:200 after 20 minutes in 4% PFA. Overall, it is recommended for Western blotting but not for that immunostaining application.
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User Reviews (3)
Review of: NMDA receptor 2B, Sigma-Aldrich, 06-600 by Peter Lund Ovesen
Does not work in immunostaining cultured hippocampal neurons.
Dilution 1:200, fixed in 4 % PFA 20 min
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0 not usefulReview of: NMDA receptor 2B, Sigma-Aldrich, 06-600 by Peter Lund Ovesen
We observed goood results for Western blotting using membrane preperation of 20 ug mouse hippocampal samples in a dilution of 1:5000. The lower antibody concentration reduced the unspecific binding, showing only the NR2B-band.
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0 useful 0 not usefulReview of: NMDA receptor 2B, Sigma-Aldrich, 06-600 by Simon Glerup
Excellent for Western blotting using membrane preparations of mouse hippocampus as samples.
Dilution 1:1000
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