LDLR, Abcam, ab14056
What do reviewers generally think about ab14056 from Abcam?
Researchers report mixed performance for LDLR, Abcam, ab14056. It works for Western blotting, detecting expected 100 kDa LDLR in mouse liver after PCSK9 administration and in 293, HepG2, mouse liver, and brain samples at 1:1000 or 1:2000, though one reviewer notes a band above 188 kDa. One reviewer points out that it fails for immunoprecipitation of endogenous LDLR from HEK293 cells, producing many unspecific bands and co-precipitating SorLA. Immunofluorescence staining of 293 cells was observed, but specificity was uncertain.
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User Reviews (3)
Review of: LDLR, Abcam, ab14056 by Camilla Gustafsen, Aarhus University
We have used this antibody for WB detection of LDLR in mouse liver after iv administration of PCSK9. The antibody detects a band of expected size (100 kDa), which is down-regulated in animals injected with PCSK9. The antibody also detects a band above the 188 kDa marker band (Western blot shown). 40 µg protein (membrane preparation) was loaded, and the antibody was diluted 1:1000 in 5% milk in TBS-T.
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0 not usefulReview of: LDLR, Abcam, ab14056 by Simon Glerup, Aarhus University
This antibody does not work for IP of endogenous LDL receptor from HEK293 cells.
We did IPs from metabolically labelled cells (S35)and saw a lot of unspecific bands. The antibody even pulled down the related receptor SorLA.
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0 useful 0 not usefulReview of: LDLR, Abcam, ab14056 by Simon Glerup, Aarhus
We use 1:2000 diluted for Western blotting of lysates of 293 and HepG2 cells, and liver and brain homogenates (mouse). Detects a band of the expected size.
It stains 293 cells when used in immunofluorescense but I´m not sure that it is specific.
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