Iba1, Abcam, ab5076
Information about this antibody
Target
Alt. Targets
Iba1
Vendor
Catalog No
ab5076
Application
Clonality
pAb
Origin
Goat
Reactivity
Human, Rat
Conjugation
N/A
Product Link
N/A
Vendor notes
No vendor notes available for this antibody.
User Reviews (2)
Review of: Iba1, Abcam, ab5076 by Jamie Rose, University of Edinburgh
Free floating sections – IHC and IF require 5% milk blocking solution and 5% citric acid antigen retrieval at 1:1000. For paraffin staining 1:1000 with 2% EDTA and 5% citric acid antigen retrieval.
IHC: 5 stars
IF: 4 stars
For more information see Pickett et al. Cell Reports 2019, “Amyloid Beta and Tau Cooperate to Cause Reversible Behavioral and Transcriptional Deficits in a Model of Alzheimer’s Disease”.
https://www.sciencedirect.com/science/article/pii/S2211124719315268?dgcid=rss_sd_all#!
Review of: Iba1, Abcam, ab5076 by Simon G, Aarhus
We tried this Iba1 antibody on PFA-fixed brain sections from mouse.
It gave very faint staining of something that might look like microglia.
I cannot recommend this antibody.
Comments