Iba1, Abcam, ab5076
What do reviewers generally think about ab5076 from Abcam?
Reviews are mixed. One reviewer reports good IHC and acceptable IF on free-floating sections at 1:1000, using 5% milk blocking and 5% citric acid antigen retrieval, and for paraffin sections at 1:1000 with 2% EDTA plus 5% citric acid retrieval, rating IHC 5 stars and IF 4 stars. One reviewer points out that on PFA-fixed mouse brain sections the antibody gave very faint staining of something that might look like microglia, and they cannot recommend it.
Information about this antibody
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User Reviews (2)
Review of: Iba1, Abcam, ab5076 by Jamie Rose, University of Edinburgh
Free floating sections – IHC and IF require 5% milk blocking solution and 5% citric acid antigen retrieval at 1:1000. For paraffin staining 1:1000 with 2% EDTA and 5% citric acid antigen retrieval.
IHC: 5 stars
IF: 4 stars
For more information see Pickett et al. Cell Reports 2019, “Amyloid Beta and Tau Cooperate to Cause Reversible Behavioral and Transcriptional Deficits in a Model of Alzheimer’s Disease”.
https://www.sciencedirect.com/science/article/pii/S2211124719315268?dgcid=rss_sd_all#!
0 useful
0 not usefulReview of: Iba1, Abcam, ab5076 by Simon G, Aarhus
We tried this Iba1 antibody on PFA-fixed brain sections from mouse.
It gave very faint staining of something that might look like microglia.
I cannot recommend this antibody.
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