TH, merckmillipore, AB152

Great antibody for IHC. Dilution used: 1:1000

For more information see García-González et al. eLife 2020, “Identification of slit3 as a locus affecting nicotine preference in zebrafish and human smoking behaviour”
https://elifesciences.org/articles/51295

I can highly recommend this antibody:

Rabbit-anti-tyrosine hydroxylase (TH; Merck Millipore, formerly Chemicon) is an outstanding primary antibody to reveal fine subcellular details of catecholaminergic neurons. Both immunoperoxidase and immunofluorescence labelling result in extremely low background staining of paraformaldehyde-fixed tissues. The properties of AB152 are exemplified for forebrain sections in rats (Riedel et al 2002, Figs 6 and 8), in mice (Michalski et al 2017, J Alzheimer Dis, Figs 3,4 and 6) and in rhesus monkeys (Brauer et al 2000, Fig 1).

Michalski et al. 2017 J Alzheimer Dis: https://www.ncbi.nlm.nih.gov/pubmed/28671120
Riedel et al. 2002: https://www.sciencedirect.com/science/article/pii/S0891061801001429?via%3Dihub
Brauer et al. 2000: https://www.sciencedirect.com/science/article/pii/S0006899300026536?via%3Dihub

The antibody was used for Immunofluorescence staining of neurons derived from murine ES cells differentiated in culture to investigate the presents of dopaminergic neurons.
The cells were grown on coated cover slips, fixed for 30min in 4% PFA and treated for 5 min with 0,005% Triton-X-100. To reduce unspecific binding cells are blocked/ quenched with a 1% BSA in PBS solution.
The Antibody was diluted 1:500. The secondary antibody was a goat anti rabbit 586 diluted 1:800.
Weak staining was observed as well as a high background. The same results were observed in double staining experiments with Sigma # T5076 mouse-anti-β-III-tub (1:8000).

Used for immunefluorescence on cryosections (10µm) of mouse embryos.
Stains very well, neurons in VTA and locus coeruleus, fibers in forebrain, sympathetic trunk neurons and adrenal medullary cells with very little background.
Dilution used was 1:250

Used for immunohistochemistry on free floating PFA fixed mouse brain sections.
Works very nicely in mice to detect dopaminergic neurons.
Dilution 1:750

Excellent TH antibody for immunohistochemistry. We even have colleagues saing that “it works like magic”.

We used it for staining TH positive fibers in the striatum and neurons in the substantia nigra and VTA in PFA fixed mouse cryosections.
Dilution 1:2000 and visualization by DAB reaction.

However, it cannot beat the one from Pel-Freez – it is the best TH-antibody for IHC