VR1, Santa Cruz Biotechnology, sc-12498
What do reviewers generally think about sc-12498 from Santa Cruz Biotechnology?
Researchers report mixed results for VR1 sc-12498. It is described as working well for immunoprecipitation and Western blotting of TRPV1 in transfected HEK293 cells, with one user noting a 1:1000 dilution and success after crosslinking. One reviewer points out that the antibody gave strong background staining in untransfected HEK293 cells and no specific signal in VR1-transfected cells. Overall, performance appears application and cell context dependent, with strong positive reports for IP and WB but a notable complaint about background and lack of specific staining.
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User Reviews (3)
Review of: VR1, Santa Cruz Biotechnology, sc-12498 by Christian Vægter
Used this ab for IP and WB, transfected cells following crosslinking experiments. Worked very well for both.
0 useful
0 not usefulReview of: VR1, Santa Cruz Biotechnology, sc-12498 by Simon Glerup
This antibody works well for IP and for Western blotting detecting TRPV1 in transfected HEK293 cells.
Dilution 1:1000
Comments
0 useful 0 not usefulReview of: VR1, Santa Cruz Biotechnology, sc-12498 by Simon Glerup
This antibody gives strong background staining in untransfected HEK293 cells. No specific signal is observed in cells transfected with VR1.
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0 useful 0 not useful
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