TH, Pel-Freeze, P40101
What do reviewers generally think about P40101 from Pel-Freeze?
Researchers describe TH, Pel-Freeze, P40101 as an excellent anti-TH antibody with very low background and superior performance to other TH antibodies. It worked well on mouse and pig brain formalin-fixed cryosections at 1:600, and on mouse brain cryosections and paraffin sections at 1:1000 for immunohistochemistry and Western blotting, including primary midbrain dopaminergic neuron cultures, giving excellent striatal TH fiber resolution. One reviewer points out that the antibody is difficult to obtain, especially in Europe, and that dealing with Pel-Freez is problematic.
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User Reviews (2)
Review of: TH, Pel-Freeze, P40101 by Trine W. Mikkelsen
We used this antobody with great succes on both mouse and pig brains (Göttingen minipig), diluted 1:600. All material was formalin-fixed cryosections. Without doubt the best anti-TH we have been using, with very low background. Unfortunately we ran out a couple of months ago, and it seems to be unobtainable in Europe. If anyone has a tip on were to find a pel-freez-distributor (DK), please add a comment with info.
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0 not usefulReview of: TH, Pel-Freeze, P40101 by Simon Glerup
Great TH antibody for Western blotting and immunohistochemistry.
Dilution 1:1000 on both cryosections and parafin sections of mouse brain.
WB dilution also 1:1000.
We have also used it on primary cultures of midbrain dopaminergic neurons.
It is far superior to all other TH antibodies that we have tested. For example it gives excellent resolution of TH fibers in the striatum.
Unfortunately, for some reason it is extremely difficult to do business with Pel-Freez and actually getting our hands on this TH antibody!
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