TrkB, Santa Cruz Biotechnology, h-181/sc-8316
What do reviewers generally think about h-181/sc-8316 from Santa Cruz Biotechnology?
Researchers generally find TrkB h-181/sc-8316 effective for Western blotting, detecting TrkB in cultured hippocampal neurons and transfected HEK293 cells at 1:500, and acceptable for IHC on PFA-fixed, cryosectioned mouse and rat brain, detecting hippocampal TrkB. Complaints include poor IP from cultured hippocampal neurons and failed immunostaining of human TrkB in 293 cells, with strong nonspecific staining. One reviewer points out that the antibody does not work very well for IP of TrkB from cultured hippocampal neurons.
Information about this antibody
Vendor notes
User Reviews (4)
Review of: TrkB, Santa Cruz Biotechnology, h-181/sc-8316 by Christian Vægter
Workes ok for IHC on mouse and rat brain sections (PFA fixed, cryosectioned). Detected TrkB in hippocampus.
0 useful
0 not usefulReview of: TrkB, Santa Cruz Biotechnology, h-181/sc-8316 by Simon Mølgaard
This antibody does not work very well for IP of TrkB from cultured hippocampal neurons. Similar it does detect trkB very well in western blots.
Dilution 1:500
Comments
0 useful 0 not usefulReview of: TrkB, Santa Cruz Biotechnology, h-181/sc-8316 by Simon Glerup
This antibody detects TrkB in transfected HEK293 cells by Western blotting.
Dilution 1:500
Comments
0 useful 0 not usefulReview of: TrkB, Santa Cruz Biotechnology, h-181/sc-8316 by Simon Glerup, Aarhus
Does not work for immunostaining of human TrkB transfected into 293 cells.
Strong unspecific staining.
Dilution 1:50
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0 useful 0 not useful
Comments